|
|
|
|
|
| ABE, Yasuyuki; LEE, Dong-Soo; SANO, Hikaru; AKIYAMA, Koji; YANAGIMOTO-UETA, Yoshiko; ASANO, Tomoyoshi; SUWA, Yoshinori; SUZUKI, Hiroshi; 鈴木, 宏志. |
| Due to the recent outbreak of avian influenza, transportation of frozen canine semen with egg yolk has been sharply restricted. Thus, there is urgent need to develop a novel egg yolk-free extender for freezing canine spermatozoa. In the present study, the effect of using skim milk/glucose (SG)-based extender without egg yolk on the motility and fertilizing capacity of canine spermatozoa frozen-thawed in the presence of glycerol was examined. There was a tendency for the proportion of motile spermatozoa exposed to SG-based extender for 3 h to be higher than that exposed for 1 h, but the difference was not significant. The motility and other viability parameters of canine spermatozoa after thawing were similar to those obtained with an egg yolk-based... |
|
Palavras-chave: Canine; Cryopreservation; Skim milk/glucose-based extender; Spermatozoa. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3030 |
| |
|
|
| ABE, Yasuyuki; SUWA, Yoshinori; YANAGIMOTO-UETA, Yoshiko; SUZUKI, Hiroshi; 鈴木, 宏志. |
| Preimplantation development of canine embryos is not well understood. To understand the timing of preattachment embryogenesis relative to the luteinizing hormone (LH) surge, early embryonic development was examined in Labrador Retrievers after artificial insemination. The embryos migrated from the oviduct to the uterus beginning on day 11 after the LH surge. This transport must be completed within 24 h. By day 13 after the LH surge, all of the embryos had moved and were localized in the uterus. The embryos developed to the morula stage within 11-13 days and to the blastocyst stage within 14 days after the LH surge, respectively. These findings add to the current understanding concerning the physiology of preimplantation development and should help further... |
|
Palavras-chave: Dog; Embryo; Preimplantation development. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3029 |
| |
|
|
|
